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The rate-limiting step in the de novo synthesis of the GSH is catalyzed by gamma-glutamyl-cysteine synthetase (also known as glutamine-cysteine ligase), which is a target gene of Nrf2

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In melanocytes, depending on the type of kinase and cell line, so far it has been proven that kinases play a dual role in Nrf2 activity regulation, e.g., increased activity of JNK (c-Jun N-terminal kinase) enhances Nrf2 expression in melanocytes line PIG1 but reducing its activation PIG3V cells 69

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