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Stability considerations in experimental design If a substantial proportion of parent peptide converts to unidentified metabolites within an hour in plasma ex vivo, several design consequences follow: Time-course design matters considerably Single-timepoint measurements may miss the effect window entirely Analytical methods separating metabolites from parent compound give a very different picture to those that do not Effects in longer experiments may be attributable to metabolites rather than the parent molecule 5

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Epub 2007 Jan 12